By E. Abuelzein
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The Vero cell toxicity test was used as a gold-standard for toxin activity. Evaluation of an Immuno-Chromatographic Detection System for Shiga Toxins and the E. coli O157 Antigen 35 In total, two strains of Shigella dysenteriae and S. sonnei (Stx1) and 18 E. coli-producing toxins of the Stx1 family (Stx1 (n=2), Stx1a (n=9), Stx1c (n=5) and Stx1d (n=4) were tested. The RIDA Quick detected all strains of the Stx1 family (sensitivity of 100%). All the reactions were 4+, except for Stx1d strains showing reaction intensities between 1+ and 3+.
Coli O104:H4 in Germany indicates that serotypes other than O157 can suddenly become the most highly virulent human pathogens (Frank et al. 2011). Healthy dairy and beef cattle are recognized as a major natural reservoir of EHEC and other STEC strains. There are more than 100 serotypes of STEC which have been also isolated * Corresponding Author 30 Trends in Immunolabelled and Related Techniques from other animals such as sheep, pigs, goats, deer, horses, dogs and birds (Gyles 2007). Humans become infected most frequently by consuming STEC-contaminated food of different kinds, but also waterborne infections and the direct transmission from STECexcreting animals or humans are frequent (Caprioli et al.
Evaluation of an Immuno-Chromatographic Detection System for Shiga Toxins and the E. coli O157 Antigen 35 In total, two strains of Shigella dysenteriae and S. sonnei (Stx1) and 18 E. coli-producing toxins of the Stx1 family (Stx1 (n=2), Stx1a (n=9), Stx1c (n=5) and Stx1d (n=4) were tested. The RIDA Quick detected all strains of the Stx1 family (sensitivity of 100%). All the reactions were 4+, except for Stx1d strains showing reaction intensities between 1+ and 3+. Similar results were obtained with the Ridascreen-Stx-EIA (Beutin et al.