By Paul M. Wassarman and Philippe M. Soriano (Eds.)
This volume comprehensively covers new technologies and methodologies that experience seemed for the learn of mouse development.
This quantity is an update of quantity 225 of MIE, ''Guide to recommendations in Mouse Development'', edited through P.M. Wassarman and M.L. DePamphilis and released in 1993. in past times 17 years many new applied sciences or methodologies have seemed for the learn of mouse improvement and this quantity comprehensively covers these, together with: new innovations for the cryopreservation of gametes and embryos, construction of transgenic and null (knockout) animals (use of ES cells), new release of conditional/inducible mutant animals, use of gene-trap mutagenesis, research of allele-specific expresion, use of recent reporter constructs, humanizing of transgenic animals, transcript profiling of mouse improvement, imaging of mouse improvement, rederivation of animals and use of mouse genomics.
Read Online or Download Guide to Techniques in Mouse Development, Part A: Mice, Embryos, and Cells, 2nd Edition PDF
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Additional resources for Guide to Techniques in Mouse Development, Part A: Mice, Embryos, and Cells, 2nd Edition
Example text
Lee, T. , Cheung, H. , and Chan, W. Y. (2010). GonadSAGE: A comprehensive SAGE database for transcript discovery on male embryonic gonad development. Bioinformatics 26, 585–586. Lewandoski, M. (2007). Analysis of mouse development with conditional mutagenesis. Handb. Exp. Pharmacol. 178, 235–262. , Brumwell, C. , Batten, D. , Norland, S. , Rice, D. , et al. (2006). BGEM: An in situ hybridization database of gene expression in the embryonic and adult mouse nervous system. PLoS Biol. 4, e86. Martinez-Morales, J.
Genome Biol. 6, R29. Heintz, N. (2004). Gene expression nervous system atlas (GENSAT). Nat. Neurosci. 7, 483. Hill, D. , Berardini, T. , Howe, D. , and Van Auken, K. M. (2010). The Gene Ontology Consortium. Representing ontogeny through ontology: A developmental biologist’s guide to the gene ontology. Mol. Reprod. Dev. 77, 314–329. Huang, D. , Sherman, B. , and Lempicki, R. A. (2009). Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 4, 44–57.
2010). Gene expression patterns during the mouse development revealed by ISH, transgenic reporter mice, and immunohistochemical staining are assembled and assigned to EMAP images. In a typical view, the original image is shown side by side with the expression pattern mapped to the whole-mount embryo and also to two-dimensional embryo sections. org 10 Thomas L. 1), the region in the mouse embryo according to its TS, or review all gene expression patterns for a specific embryo stage to see which expression domains might match the user’s experimental results.