By Paul D. Boyer (Eds.)
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IROSOMALBINDINGO F fMgT Factor additions Experiment Temp. 5 From Chae el al. (54). 5 A l W units). Incubation 15 min. c Net values (blanks with no added factors subtracted) in picomoles per sample. 0 b TABLE V AUG-DEPENDENT BINDING OF fMET t,ltNA WITH RInosoMAL 0' AND 2 5 " " ~ ~ N SUBUNITS A T Factor additions (pg) Temp. 0 From Chae et al. (64). 6 A*@units) were used. As assayed by poly U-dependent polyphenylalanine synthesis the contamination of 30 S with 50 S subunits waa about 10%. Net values (blanks with no added factors subtracted) in picomoles per sample.
Pro1 Fed. Amer. Exp. Biol. 31,410 (1972). 50. J. L. Fakunding and J. W. B. Hershey, JBC 248, 4206 (1973). 51. R. Benne, N. Naaktgeboren, J. Gubbens, and H. 0. Voorma, Eur. J. Bioche 32, 372 (1973). 52. A. H. Lockwood, P. Sarkar, and U. Maitra, Proc. Nnt. Acad. Sci. U . S. 3602 (1972). 1. POLYPEPTIDE CHAIN INITIATION 15 added GTP if stoichiometric amounts of IF-2 are present (53). The postulated role of IF-2-fMet-tRNAf-GTP as an obligatory in v i m intermediate in formation of the chain initiation complex (47, &), is, therefore, open to question.
B. Keller, Proc. N a t . Acad. Sci. U . S. 67, 1681 (1970) 105. S. N. Seal, J. D. Bewley, and A. Marcus, JBC 247, 2592 (1972). 106. S. N. Seal and A. Marcus, BBRC 46,1895 (1972). 107. D. P. Leader and I. G.