By Phillip E. Patton, David E. Battaglia
A finished and practice-oriented source consultant to presently on hand diagnostic and therapies for male infertility problems. subject matters lined diversity from uncomplicated sperm biology and male reproductive endocrinology, to immunology, really good sperm trying out, and the genetic historical past to male infertility. The authors emphasize the research, diagnostic checking out, and administration of the infertile male, but additionally research such well timed matters as gender choice, HIV discordance undefined, and posthumous replica. different subject matters of curiosity contain laboratory accreditation, vasectomy reversal, moral and felony concerns of donor insemination, optimizing good fortune in a donor insemination application, and strategic treatments for ejaculatory issues and erectile disorder in infertile males.
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Sample text
J Urol 1980;124:464. 49. Griffin JE, McPhaul MJ, Russell DW, Wilson JD. The androgen resistance syndromes: Steroid 5-alpha-reductase 2 deficiency, testicular feminization, and related disorders. In: Scriver Endocrinology of Male Infertility 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 37 CR, Beaudet AL, Sly WS, Valle D, eds. The Metbolic and Molecular Bases of Inherited Disease, 8th edition. McGraw-Hill, New York, NY, 2001, p. 4117. Christensen RB, Matsumoto AM, Bremner WJ.
Generally, the specimens are examined at × 20 or × 40 magnification. Depending on the methodology employed (see Chapter 9), an appropriate counting chamber (Makler Chamber, disposable counting slide, hemocytometer) is used to perform the sperm count and motility (11). General laboratory supplies are also required to prepare semen for insemination. Sterile-graduated pipets, able to measure 1, 5, and 10 mL can be used or sterile 1- to 2-mL transfer pipets. Transfer pipets are equipped with a suction bulb, whereas the sterile-graduated pipets require a mechanical bulb or electric pipet-aid to aspirate the specimen into the pipet.
This incubator can also be used to hold the semen specimen during liquefaction time prior to initiation of processing and to hold the final prepared specimen before insemination. A small clinical centrifuge is needed to process the specimens and must be able to spin the tubes at approx 300g. Various types and models of centrifuges are available—some with fixed speeds and others with adjustable speeds. A timer is also essential because the centrifugation procedure is timed at each step of the processing protocol.