By Adolf E. Schindler Prof. Dr. med. (auth.)
This monograph represents the 1st complete evaluate of hormones in human amniotic fluid and comprises facts released as much as and together with 1980. lately, extra large use of amniocentesis for prenatal prognosis and overview of fetal lung maturation has proven that amniotic fluid hormone measurements can relief within the prognosis of fetal and placental abnormalities. the fabric is gifted in major sections facing steroid and protein hormones. The tools of identity and quantitation are delineated, and the findings are mentioned with regards to the scientific stipulations. additionally, specific realization has been directed in the direction of up to date evaluation of the resources, metabolism and move of human amniotic fluid hormones. The assessment is meant to serve the desires of clinicians, simple scientists and scholars, delivering distinct info on human amniotic fluid hormones so one can increase sufferer care and point out probabilities for extra investigations. Ttibingen, January 1982 A.E. Schindler Contents creation A. 1 starting place of Human Amniotic Fluid . 2 B. C. foundation and law of Steroids in Human Amniotic Fluid . five D. equipment of Isolation and identity of Steroids in Human Amniotic Fluid 6 I. C , C , and C Steroids . 6 30 29 28 II. C Steroids 6 27 1. ldl cholesterol 6 2. Cholestanol . 6 three. ,::l7 -Cholestenol and ,::l8 -Cholestenol. 6 four. 7-Dehydrocholesterol and Desmosterol . 6 ,::l5_C Steroids. III. 7 21 1. Pregnenolone 7 2. 16cx-H ydroxypregnenolone. 7 three. 17cx-Hydroxypregnenolone.
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Dihydroxycortisone The data available so far are summarized in Table 29. Only in anencephaly are lower values evident. Similar data for normal term deliveries were also described by Drafta et al. [108], and in one case of anencephaly this steroid was not detectable. Table 29. 5 8. C 21 Steroids Only few data are available so far. Lg/liter [66]. Lg/liter (mean ± SD) [66]. Lg/liter for the sulfate. Lg/iiter was found with no correlation to gestational age [71]. Lg/liter, respectively [66]. Lg/iiter [66].
Further data have been obtained by a recent study [79] as listed in Table 35. Table 35. 0 ILg/liter; THS: 89 and 189 ILg/liter. Thus, only THS was significantly higher in amniotic fluid when compared to normal controls. A significantly elevated THFT:iHE ratio was also found in pregnancies with affected fetuses [79]. The data suggest that the diagnosis of 11~-hydroxylase deficiency may be established more precisely during pregnancy than within the first 2 weeks of gestation if there are no clinical features of the disease in the newborn [79].
I Cl) c: Cl) 60 Vi e 50 -0 c: « I 40 I:> u-> 30 , 20 10 Fig. 10. 5-Androstene-3~,17a-diol levels in amniotic fluid in some cases of pathologic pregnancy. (Mancuso et aI.