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Additional info for Carbohydrate Metabolism, Part B
Example text
1 M LiCl without Na2CO~ (D). (A), (B), and (C) were recorded, respectively, 7, 25, and 80 min after the addition of Na2CO3. 25 gA/V. In the derivative polarograms (. . ), only the midpoint of each oscillation (D = 8) is shown. (C) The way the diffusion current can be calculated. Current i s stands for the limiting current at pH 7. The second wave in (D) disappears in alkali and is of no interest here. Modified from J. Van Beeumen and J. De Ley, Anal. Biochem. 44, 254 (1971). ' T h e excess needed is inversely proportional to the extent of hydration.
3 is missing when an excess of boric acid or tetraborate has been added to the 3-ulose, prior to the Na2CO3. This phenomenon is very likely due to complex formation of the hydrate with 4j. Van Beeumen and J. De Ley, Bull. Soc. Chim. Belg. 80, 683 (1971). ", " ' ; i I I U I I J i i i I . 1 I i I I . 2 -I,6 I , . 0 (Volt) FIG. 1. 1 M LiCl without Na2CO~ (D). (A), (B), and (C) were recorded, respectively, 7, 25, and 80 min after the addition of Na2CO3. 25 gA/V. In the derivative polarograms (. . ), only the midpoint of each oscillation (D = 8) is shown.
0 ml) is added, and the tubes are kept for 10 min in a boiling-water bath. 0 ml) are then added immediately, without cooling. The solutions are stirred vigorously with a Vibromixer; after 5 min in the dark, the absorbance at 595 nm of the violet Fe (TPTZ)~ + is read against a reagent blank. Comments The color produced is stable for at least 6 hr when the mixture is kept in the dark, and also when it is diluted with several volumes of water. 3 mole equivalents of Fe(TPTZ)~ + (cf. 4). 2 %. Samples taken from biological solutions after deproteinization with trichloroacetic acid or perchloric acid can be assayed without difficulty.