Glycomics by Minoru Fukuda

By Minoru Fukuda

Some time past decade, there was an explosion of growth in knowing the jobs of carbohydrates in organic structures. This explosive growth used to be made with the efforts in deciding on the jobs of carbohydrates in immunology, neurobiology and lots of different disciplines, studying each one targeted procedure and using new expertise. This quantity represents the second one of 3 within the equipment in Enzymology sequence, together with Glycobiology (vol. 415) and practical Glycomics (vol. 417), devoted to disseminating info on equipment in picking out the organic roles of carbohydrates. those books are designed to supply an creation of latest how to a wide number of readers who wish to perform and give a contribution to the development of glycobiology. The tools lined comprise structural research of carbohydrates, organic and chemical synthesis of carbohydrates, expression and backbone of ligands for carbohydrate-binding proteins, gene expression profiling together with micro array, and iteration of gene knockout mice and their phenotype analyses.

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This observation raises an interesting question as to how EXTL2 determines its donor substrate specificity towards the N‐acetylhexosamines. , 2005). ITC directly measures the heat evolved or absorbed as a result of a given binding process. An ITC instrument essentially consists of a sample cell, a reference cell, and an injecting syringe. In an ITC experiment, the METHODS IN ENZYMOLOGY, VOL. 1016/S0076-6879(06)16001-2 4 carbohydrate–protein interactions–physical measurements [1] solution containing the protein or enzyme of interest is loaded into the sample cell of the instrument and the ligand solution is placed into the injecting syringe.

2005). ITC directly measures the heat evolved or absorbed as a result of a given binding process. An ITC instrument essentially consists of a sample cell, a reference cell, and an injecting syringe. In an ITC experiment, the METHODS IN ENZYMOLOGY, VOL. 1016/S0076-6879(06)16001-2 4 carbohydrate–protein interactions–physical measurements [1] solution containing the protein or enzyme of interest is loaded into the sample cell of the instrument and the ligand solution is placed into the injecting syringe.

JOHNSON VOLUME 385. Imaging in Biological Research (Part A) Edited by P. MICHAEL CONN VOLUME 386. Imaging in Biological Research (Part B) Edited by P. MICHAEL CONN VOLUME 387. Liposomes (Part D) Edited by NEJAT DU¨ZGU¨NES, VOLUME 388. Protein Engineering Edited by DAN E. ROBERTSON AND JOSEPH P. NOEL VOLUME 389. Regulators of G-Protein Signaling (Part A) Edited by DAVID P. SIDEROVSKI VOLUME 390. Regulators of G-Protein Signaling (Part B) Edited by DAVID P. SIDEROVSKI METHODS IN ENZYMOLOGY xxxvii VOLUME 391.

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