By Juha P. Kokko, Richard L. Tannen
This version offers an authoritative method of sufferers with fluid electrolyte and acid-base issues. It presents either a close matrix for coping with sufferers with particular electrolyte issues and medical stipulations linked to electrolyte imbalances. It includes new fabric on issues of being pregnant, overall physique sodium, water metabolism and scientific meals. Diagnostic and healing techniques in addition to pathophysiology are precise.
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Example text
M. DiGirolamo, and F. M. Huennekens, Mol. Pharmacol. 11, 174 (1975). 7 D. W. Jacobsen, R. J. Holland, Y. D. Montejano, and F. M. Huennekens, J. lnorg. Biochem. 10, 53 (1979). 8 D. W. Jacobsen, this series, Vol. 67, Part F, p. 12. 9 D. W. Jacobsen, Y. D. Montejano, and F. M. Huennekens, Anal. Biochem, 113, 164 (1981). 10 H. P. C. Hogenkamp and J. E. Rush, Biochem. Prep. 12, 121 (1968). tl W. Friedrich and K. Bernhauer, Chem. Ber. 89, 2507 (1956). lz R. L. Blakley, J. Biol. Chem. 240, 2173 (1965).
32/min. F r o m J a c o b s e n et al. 8 [4] AFFINITY PURIFICATION OF BI2-BINDING PROTEINS 33 suction filtered through a layer of cotton gauze over Whatman No. 4 paper. Pepsin-inactivated human gastric juice and human saliva are thawed and filtered in a similar fashion. Extracts from rat stomachs are prepared by homogenization in a blender followed by centrifugation at 4° until a clear supernatant is obtained. 5% Triton X-100 and debris is removed by centrifugation. 5) containing I mg/ml bovine serum albumin to an approximate UCBC of 5 ng CN-Cbl/ml or less.
Huennekens, J. lnorg. Biochem. 10, 53 (1979). 8 D. W. Jacobsen, this series, Vol. 67, Part F, p. 12. 9 D. W. Jacobsen, Y. D. Montejano, and F. M. Huennekens, Anal. Biochem, 113, 164 (1981). 10 H. P. C. Hogenkamp and J. E. Rush, Biochem. Prep. 12, 121 (1968). tl W. Friedrich and K. Bernhauer, Chem. Ber. 89, 2507 (1956). lz R. L. Blakley, J. Biol. Chem. 240, 2173 (1965). [2] ANALYSIS OF CORRINOIDS BY H P L C 17 100/zg/ml). Cells were grown to late-log phase ( - 2 × 106 cells/ml) for 48 hr at 37° in a humidified atmosphere of 95% air-5% CO2.