By Ivor Smith
Chromatographic and Electrophoretic strategies, quantity II: quarter Electrophoresis, moment version offers attempted and verified equipment of area electrophoresis, which has been performed on commercially to be had gear providing many merits to nearly all of laboratories.
This booklet is split into 14 chapters and starts with a short creation to the final ideas of area electrophoresis. the following chapters take care of the rules, instrumentation, and functions of varied tools of region electrophoresis, together with low- and high-voltage paper, cellulose-acetate, thin-layer, agar- and starch-gel, and preparative block electrophoresis. the remainder chapters conceal different sector electrophoresis equipment, akin to acrylamide gel, disc, acrylamide flat gel, and non-stop electrophoresis.
This booklet is of worth to analytical chemists and biologists.
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Extra info for Zone Electrophoresis. Chromatographic and Electrophoretic Techniques
Example text
The ninhydrin colours can be rendered permanent as described on p. 96, Vol. I. Other Reagents and Techniques. Most of the reagents described on pp. 96-102, Vol. I, for aminoacids are appHcable here. Oxidation of the sulphur-containing aminoacids is useful but the copper carbonate technique is not applicable as the copper complexes are not stable in the acid or alkahne buffers. Results Obtained with Synthetic Mixtures of Aminoacids Only the acidic, basic and /S-aminoacids, and cystine can be separated completely from a complex mixture by using the various buffer solutions (see Fig.
H . Ann. Hum. , L o n d o n . 1959, 2 3 , 442. PAPER ELECTROPHORESIS AT LOW VOLTAGE 35 16. B l a s s , J . , a n d R e b e y r o t t e , P . Bull. Soc. Chim. , P a r i s , 1953, 3 5 , 953. 17. B r o n k , J . , a n d Fisher, R. B . Biochem. J . , 1956, 6 4 , 106. 18. S m i t h , J . D . in The Nucleic Acids, Vol. 1. p . 267, A c a d e m i c Press, N e w York, 1955. 19. D a v i d s o n , J . , a n d Smellie, R . M. S. Biochem. , 1952, 5 2 , 294. CHAPTER 2 HEMOGLOBINS Jacqueline B. Weiss THE separation and identification of variants of normal haemoglobin are becoming increasingly important not only in the clinical diagnosis of hsemoglobinopathies but also in the study of genetics and anthropology.
I Paper electrophoresis or resin chromatography. See H b Η . H 8-6 these are the t w o fastest moving haemoglobins. On resin chromato graphy runs between H b Ν and H b F . J Paper electrophoresis. Electrophoreticaily runs slightly slower than H b Ν at ρΉ. 8-6. A t ρΈί β·5 no separation takes place but H b s A + Ν separate. Chro matographically no separation from H b A occurs. ) Κ Paper electrophoresis. Electrophoreticaily runs faster than H b A at ρΉ. 8-6. May be very slight separation from H b A at ρΉ.