By G.N.Hatsopoulos
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Botulinum Strains by HPHT Processing Until now, the resistance to pressure and temperature has been studied with at least five C. , 2004; Gola and Rovere, 2005). C. , 1999). Furthermore, C. , 2003). Other studies on several C. botulinum strains (types A, B, F proteolytic, B non-proteolytic) in mashed carrots were carried out by Margosch et al. (2004) and Margosch (2005). 5 log cycles to none at all. In particular, non-proteolytic C. 5 log cycles after 80°C/600 MPa/1 s. In comparison, proteolytic C.
5 require refrigerated storage and other preservation hurdles to prevent enzymatic degradation reactions and to inhibit spore germination. , 1970; Heinz and Knorr, 2001). Furthermore, inactivation data on C. , 1999; Heinz and Knorr, 2001), where pressures in the range of 690 to 900 MPa were combined with initial temperatures between 50 and 70°C. The following section will provide an in depth description of the potential application of high pressure combined with heat in commercial sterilization.
The outer cylinders compress the inner cylinders such that the wall of the pressure chamber is always under some residual compression at the design operating pressure. In some designs, cylinders and frame are prestressed by winding layer upon layer of wire under tension. The tension in the wire compresses the vessel cylinder so that the diameter is reduced (Hjelmqwist, 2005). This special arrangement allows an equipment lifetime of over 100,000 cycles at pressures of at least 680 MPa. The preferred practice is to design high-pressure chambers with stainless steel food-contacting parts so that filtered (potable) water can be used as the isostatic compression fluid (Farkas and Hoover, 2000).