By John L. Curtis
This finished, step by step laboratory education guide brings the entire parts for a profitable embryo move application jointly in an easy, prepared, illustrated structure. For the final numerous a long time, man made insemination has allowed genetic development to be completed particularly fast in the course of the frequent and effective use of frozen semen. as a result development of embryo move (ET) suggestions, cows can produce many offspring. A extra swift genetic achieve is completed which enhances a synthetic insemination software.
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Additional info for Cattle Embryo Transfer Procedure: An Instructional Manual for the Rancher, Dairyman, Artificial Insemination Technician, Animal Scientist, and Veter
Sample text
6. Prepare post-collection infusion aild Estrumate. 7. Administer epidural anesthesia; wash vulva. 8. Suspend PBS; fill Em-Con filter half-full. 3) EMBRYO COLLECTION At this point, it is convenient to have an assistant available for catheter inflation, stylet removal, and possible manipulation of the Em-Con filter. The sequential steps for embryo collection are: Pull on a shoulder-length sleeve (fingertips removed) and then a latex examination glove. Using a hemostat, clamp the open end of the sleeve to your shirt at the shoulder.
Embryos are frequently located in the oviducts prior to day 6 and therefore cannot be collected by the nonsurgical recovery technique. 5, the clear sphere (zona pellucida, Figures 12 and 13) surrounding the embryonic cellular mass has ruptured, leaving an embryo too mature for freezing. Pregnancy rates from fresh (not frozen) embryos collected and transferred 6 to 8 days after heat are not different. However, in order to correctly freeze embryos produced in excess of recipients on a particular day, collection should be scheduled for day 7.
Figure 28. Foley clamped to the stylet, ready for insertion. By: Mohamed Abd El Wahab 44 Cattle Embryo Transfer Procedure . plished by epidural anesthesia, which should be administered at this time. Scrub the donor's tail-head with Betadine scrub and rinse with water. 5 inch needle). Grasp the tail firmly ('ust below the rectum) and elevate it 130 to 140" straight up and forward, applying constant forward pressure. Using the thumbnail of your free hand, press down firmly on the scrubbed tail-head region to locate an indentation between two vertebrae.