Advanced Techniques in Biological Electron Microscopy II: by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)

By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)

The use of the time period "advanced" within the identify of this publication is a little ar­ bitrary and intensely a lot relative with recognize to time. Many strategies that have been thought of on the "cutting part" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in quite a few laboratories. you possibly can cite freeze-fracture, cryothin sectioning, or certainly lots of the box of experiment­ ning electron microscopy as concrete examples. hence using the time period "ad­ vanced ideas" has to be interpreted in regards to the current cutting-edge, and turns out to be useful in basic terms in informing the aptitude reader that this quantity isn't really a primer for use as an preliminary creation into uncomplicated organic elec­ tron microscopy. many fantastic volumes have stuffed that area of interest long ago few years, and it isn't meant that this modest publication be an entire com­ pendium of the sector. additionally, any restricted collection of papers on advanc­ ed strategies unavoidably displays the personal tastes and arbitrary whims of the editor, thereby aside from many both very important tactics which the an expert reader will without problems determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated options that have been idea to symbolize complex and but ba­ sically morphological equipment for gaining elevated ultrastructural informa­ tion from organic specimens. the current quantity, nonetheless, stresses options which offer particular physicochemical facts at the speci­ mens as well as the structural information.

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BioI. Chern. 249, 803 - 810 (1974) Olson, M. , Liener, I. : The association and dissociation of concanavalin A, the phytohemagglutinin of the jack bean. : A two-stage method for cross-linking antibody globulin to ferritin by glutaraldehyde. ], Immunol. Meth. 3, 137 - 146 (1973) Parmley, R. , Spicer, S. ]. Hisrochem. Cytochem. , Kostir,]. : Studies on phytohaemagglutinins. VII. Effect of Mn 2+ and Ca 2+ on haemagglutination and polysaccharide precipitation by phytohaemaggl~tination of Pisum sativum.

Schematic 'glycograms' representing the different rates of redistribution and internalization of the cells labeled and shown in Figure 5. N: Normal secondary hamster embryo cells. T: SV-40 transformed hamster cells. (From HUET and BERNHARD, 1974, courtesy of Dr. C. HUET) 0° C for 2 h followed by peroxidase labeling at 37° C for 15 min results in dramatic rearrangement and endocytosis (Fig. 7 c), if the Con A-peroxidase labeling is performed as usual at 22° C, a patchy distribution of Con A receptors ,is obtained (Fig.

Cell BioI. , Douglas, S. : Localization of Al antigen sites on human erythrocyte ghosts. : Membrane structure and surface coat of Entamoeba histolytica. J. Cell BioI. , Nicolson, G. : Freeze-etch localization of concanavalin A receptors to the membrane intercalated particles on human erythrocyte membranes. Biochim. Biophys. Acta 363, 311 - 319 (1974) Poretz, R. , Goldstein, I. : The hydrophobic character of phenyl glycosidases and its relation to the binding of saccharides to concanavalin A. Arch.

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